ABSTRACT
Objective To analyze the relationship between the genetic polymorphisms of organic anion transporting polypeptide (SLCO1B1 and SLCO1B3) and mycophenolic acid ( MPA)pharmacokinetics in Chinese kidney transplant recipients. Methods Gene mutations (SLCO1B3T334G, SLCO1B1 A338G) were detected in 68 recipients by PCR-LDR. The plasma samples were collected and blood concentration of MPA was measured on the 28 th day after transplantation. The area under the curve (AUC)0-12 of MPA in different genotype recipients was compared to analyze the correlation between single nucleotide polymorphisms (SNPs) and MPA pharmacokinetics. Results MPA AUC0-12 was higher in SLCO1B3 T334G GG carriers group than in TT carriers [(54. 54 ±14.40)vs(37.30±12.88)mg·h·L-1,(P=0.052)].However,there was no difference in MPA AUC0-12 among each genotype of SLCO1B1 A338G (P>0. 05). Conclusion Genetic polymorphisms of SLCO1B3 affect interindividual variety in plasma MPA concentration in Chinese kidney transplantation recipients.
ABSTRACT
Myocardial fibrosis caused by various reasons (such as inf la mmatory, ischemia, hypertension) can induce the myocardial damage if the process continued. There is very progress in the research of the prevention and cure of myocardial fibrosis in present years. This paper summarizes the detection of my ocardial fibrosis in laboratory, and the progress and mechanisms of medication i n myocardial fibrosis.
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Aim To explore the role of macrophages in the progression of fibrosis in spontaneously hypertensive rats and study effects and possible mechanisms of benazepril.Methods 12-week-old male SHRs were divided into hypertension group(SHR,n=10),benazepril treatment groups(BenL,BenH subgroup;Rats received benazepril 1.7 or 17 mg?kg-1?d-1 intragastric administration,respectively;each n=10),and age and sex matched Wistar Kyoto rats were set as control group(WKY,n=10).After 18 weeks' intervention,systolic blood pressure(SBP),left ventricular mass(LVM),left ventricular mass index(LVMI),contents of left ventricular collagen and macrophages were examined.Transcriptional levels of TGF-?_1 and interleukin-6(IL-6)were evaluated by reverse transcription polymerase chain reaction(RT-PCR).Results The amount of macrophages in SHR group was strikingly increased and transcriptional levels of TGF-?_1 and IL-6 in SHR group were much higher when compared with WKY group(P